A small number of votes for Reform could mean big wins for the SNP, the Scottish Conservatives have said.
Party chairman Craig Hoy said there was a huge opportunity to beat the SNP – but it could be lost if voters chose to back Reform.
Polls show that a small number of votes for Reform could help the SNP to win several seats.
Several Reform candidates in Scotland are also pro-independence.
Gordon and Buchan candidate Kris Callander and Dundee Central candidate Vicky McCann both supported independence.
Reform’s deputy chairman, David Kirkwood, is another independence voter. He is standing in the Dumfriesshire, Clydesdale and Tweeddale seat.
When asked directly last week, Reform chairman Richard Tice couldn’t say how many of his candidates are pro-independence.
Scottish Conservative party chairman, Craig Hoy, said: “Many seats in Scotland are a straight contest between the SNP and Scottish Conservatives.
“In those key seats, no other party can win. Reform, Labour and the Lib Dems are simply too far behind.
“Even a small number of votes for Reform could lead to big wins for the SNP.
“The opportunity is within our grasp to hand the SNP their worst election defeat in more than a decade, but just a few people choosing to vote Reform could put all that at risk.
“In key seats up and down Scotland, only a vote for the Scottish Conservatives can beat the SNP and get the focus onto the issues that really matter to you.”